UMass Amherst

Welcome to the home page of BMB 695a,
Macromolecular Crystallography (Spring 2008)!

The UMass protein crystallography home page is at

This journal club is organized by Scott Garman, Jeanne Hardy, and Karsten Theis.We share a common interest in structural biology, including X-ray crystallographic techniques. Our labs are all located on the 10th floor of Lederle Tower, along with the X-ray diffraction facility.

If you are looking for the home page of the UMass X-ray diffraction facility, you can find it here.

If you are looking for Karsten Theis' home page, it is here, while Scott Garman's home page is here, and Jeanne Hardy's is here.

Spring 2008 Journal Club Schedule (Biochem 695a, Macromolecular Crystallography):

Journal Club meets Fridays at 9:00 in 1020 LGRT (Pinkham room). Every week, we will have one discussion leader who will ask for multiple volunteers to present selected data (figures or tables).

Readings and Links
8 Feb 08
Polarity protein Sro7 Cassidy Paper, Suppl
Douglas A. Hattendorf, Anna Andreeva, Akanksha Gangar, Patrick J. Brennwald & William I. Weis,
Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism, Nature (2006) 446, 567-571
15 Feb 08
Auxin receptor
Paper, Suppl
Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N.
Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature (2007) 446:640-645
22 Feb 08
Follow up Cassidy See the two papers above
We will finish up some loose ends from the first two weeks: how the structures were solved, how to read Table I, etc.
29 Feb 08
Na+,K+-ATPase Sravanti Paper, Suppl
J. Preben Morth, Bjørn P. Pedersen, Mads S. Toustrup-Jensen, Thomas L.-M. Sørensen,
Janne Petersen, Jens Peter Andersen, Bente Vilsen, & Poul Nissen
Crystal structure of the sodium–potassium pump. Nature (2007) 450:1043-1050
7 Mar 08
ABC transporter Mary Paper, Suppl

Michael L. Oldham, Dheeraj Khare, Florante A. Quiocho, Amy L. Davidson & Jue Chen
Crystal structure of a catalytic intermediate of the maltose transporter, Nature 450, 515-521(22 November 2007) 

14 Mar 08
Flu M2 (X-ray) Mike Paper, Suppl, N&V
Amanda L. Stouffer, Rudresh Acharya, David Salom, Anna S. Levine, Luigi Di Costanzo, Cinque S. Soto,
Valentina Tereshko, Vikas Nanda, Steven Stayrook & William F. DeGrado
Structural basis for the function and inhibition of an influenza virus proton channel. Nature (2008) 451:596-600 
Spring Break
28 Mar 08
Flu M2 (NMR) Sam Paper, Suppl
Structure and mechanism of the M2 proton channel of influenza A virus
Jason R. Schnell & James J. Chou. Nature (2008) 451:591-596 
4 Apr 08
G protein coupled receptor Mylene Paper, Suppl
High-Resolution Crystal Structure of an Engineered Human β2-Adrenergic G Protein–Coupled Receptor
Vadim Cherezov, Daniel M. Rosenbaum,... Raymond C. Stevens, Science (2007) 318:1258 - 1265
11 Apr 08
GPCR function Kristen  
GPCR Engineering Yields High-Resolution Structural Insights into β2-Adrenergic Receptor Function
Daniel M. Rosenbaum, Vadim Cherezov,...Brian K. Kobilka, Science (2007) 318:1266 - 1273
18 Apr 08
PIP2-Kinase Hao Paper, Suppl
The Structure of a Human p110/p85 Complex Elucidates the Effects of Oncogenic PI3K Mutations.
Chuan-Hsiang Huang, ... L. Mario Amzel, Science (2007) 318: 1744-1748
25 Apr 08
Intramembrane Protease Nilima Paper, Suppl
Crystal structure of a rhomboid family intramembrane protease.
Yongcheng Wang, Yingjiu Zhang, & Ya Ha, Nature (2006) 444:179-183.  
2 May 08
Chang papers 1 Witold MsbA paper, suppl; MsbA complex paper, suppl;
EmrE paper, suppl
9 May 08
Chang papers 2 Abby Science Compass 2001, Crosslinking 2003;
PNAS MsbA corrected structures paper, suppl